Method for detecting biological activity of recombinant human erythropoietin (rhEPO) by RGA technology

The invention discloses a novel method for detecting biological activity of recombinant human erythropoietin (rhEPO) by a RGA technology. The basic principle of the method comprises that through STAT5 reaction components (comprising sis inducible element (SIE), the gammaresponse region (GRR) and the interferon gamma-activated sequence (GAS)), a reporter gene vector is constructed, UT-7 cells are subjected to transfection, a stable monoclonal cell strain UT-7/G7 is obtained by pressurization screening and monoclonal separation cultivation, after rhEPO and a ligand are bonded, through a series of signal transduction, expression of reporter gene luciferase is activated, and through detection of a change of luciferase expression after rhEPO stimulation, rhEPO activity is quantified. The method concretely comprises the following steps of washing UT-7/G7 cells by DPBS three times, carrying out culture by an analysis medium for 17-20h, adding rhEPO solutions having a series of concentrations into a 96-well plate hav