Pseudosciaena crocea fish scale oxidation-resistant collagen peptide, and preparation method and application thereof
The invention discloses a Pseudosciaena crocea fish scale oxidation-resistant collagen peptide, and a preparation method and application thereof. The amino acid sequence of the oxidation-resistant collagen peptide is Gly-Pro-Thr-Gly-Phe-Pro-Gly (SEQ ID No:1), and the molecular weight by ESI-MS detec...
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Zusammenfassung: | The invention discloses a Pseudosciaena crocea fish scale oxidation-resistant collagen peptide, and a preparation method and application thereof. The amino acid sequence of the oxidation-resistant collagen peptide is Gly-Pro-Thr-Gly-Phe-Pro-Gly (SEQ ID No:1), and the molecular weight by ESI-MS detection is 631.70Da ([M+H]+632.49Da). The method comprises the following steps: by using Pseudosciaena crocea fish scale as a raw material, adding 2-3% hydrochloric acid according to the solid-to-liquid ratio of 1g:(10-15mL), treating for 20-24 hours, filtering to remove the hydrochloric acid, adding 0.08-0.10 mol/L NaOH according to the solid-to-liquid ratio of 1g:(10-15mL), treating for 20-24 hours, filtering, washing with distilled water until the pH value is 6.5-7.5, drying at 30-35 DEG C, and pulverizing with a pulverizer; and taking the fish scale powder, adding a glycine-NaOH buffer solution according to the material-to-liquid ratio of 1g:(8-10mL), regulating the pH value to 9.0-10.0, heating the enzymolysis so |
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