Pseudosciaena crocea fish bone collagen peptide, and preparation method and application thereof

The invention discloses a Pseudosciaena crocea fish bone collagen peptide, and a preparation method and application thereof. The amino acid sequence of the collagen peptide is Gly-Phe-Pro-Gly-Ser-Phe-Arg (SEQ ID No:1), and the molecular weight by ESI-MS detection is 823.92Da ([M+H]+824.77Da). The me...

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Hauptverfasser:	CHEN YIN, CHI CHANGFENG, HU FAYUAN, WANG BIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a Pseudosciaena crocea fish bone collagen peptide, and a preparation method and application thereof. The amino acid sequence of the collagen peptide is Gly-Phe-Pro-Gly-Ser-Phe-Arg (SEQ ID No:1), and the molecular weight by ESI-MS detection is 823.92Da ([M+H]+824.77Da). The method comprises the following steps: chopping Pseudosciaena crocea fish bone, removing non-collagen substances, carrying out decalcification and degreasing treatment, and preparing fish bone collagen by acid extraction and NaCl salting-out; adding the prepared fish bone collagen into distilled water according to the material-to-liquid ratio of 1g:(15-20mL), regulating the pH value to 1.2-2.0, keeping the temperature at 35-40 DEG C for 5-10 minutes, adding pepsin which accounts for 1.0-1.5 wt% of the collagen, and carrying out enzymolysis for 4-6 hours; regulating the temperature of the enzymolysis solution to 40-50 DEG C, regulating the pH value to 9.0-10.0, keeping the temperature for 5-10 minutes, adding alkaline