Method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) by using silver ion modified amino silica gel

The invention discloses a method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) by using silver ion modified amino silica gel. According to the method, the silver ion modified amino silica gel is taken as a stationary phase to fill a chromatographic column, and base line separation of EPA and DHA is realized by changing the proportion of amino silica gel and silver nitrate, an elution system and the sampling amount. A filler prepared with the method effectively separates EPA and DHA, meanwhile, the problems of instability of silver ions on silica gel and the sample pollution caused by the fact that silver ion is easy to fall off in the separating process are solved, and the column utilization rate is increased.