UDP-glucuronic acid decarboxylase gene function detection

The present invention discloses a UDP-glucuronic acid decarboxylase (UXS) gene function detection. According to the present invention, recombinant plasmid of a UXS6 gene and an expression vector pEGX-4T-2 is constructed and is transformed into bacteria to carry out prokaryotic expression, function d...

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Bibliographische Detailangaben
Hauptverfasser: ZHOU CHUN, FAN JING, FU HAOYANG, KUANG BEIQING, WU AIMIN, NI CHUNXU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present invention discloses a UDP-glucuronic acid decarboxylase (UXS) gene function detection. According to the present invention, recombinant plasmid of a UXS6 gene and an expression vector pEGX-4T-2 is constructed and is transformed into bacteria to carry out prokaryotic expression, function detection is performed by using a high performance liquid chromatographic instrument after the UXS6 protein reacts with uridine diphosphate glucuronic acid, and the optimum conditions of the enzyme activity reactions at different temperatures under different pH values are determined; the recombinant plasmid is adopted to transform tobacco to carry out over-expression so as to further study the effect of the UXS in plants, and uridine diphosphate xylose is produced after the reaction, and is the substrate of hemicelluloses xylan so as to provide important application values in industry; and the artificially reliable way is provided for industrial requirements, and the glucuronic acid gene in the plant can be silenced