Endophytic fungus strain having high paclitaxel yield and method for producing paclitaxel by endophytic fungus strain
The invention discloses an endophytic fungus strain, which is capable of producing paclitaxel and is separated from young stems of taxus chinensis. The endophytic fungus strain is named as Botryosphaeria dothidea J11, is preserved in the China center for type culture collection and has an accession...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses an endophytic fungus strain, which is capable of producing paclitaxel and is separated from young stems of taxus chinensis. The endophytic fungus strain is named as Botryosphaeria dothidea J11, is preserved in the China center for type culture collection and has an accession number of CCTCC NO: M2013591. The invention also relates to a method for producing paclitaxel by the preserved endophytic fungus strain. The method comprises the following steps of culturing the preserved endophytic fungus strain by a culture medium so that paclitaxel is produced and gathered in strain hyphae, recovering the paclitaxel in the cells and carrying out purification. The endophytic fungus strain provides a novel strain for microbial fermentation production of paclitaxel. Through the endophytic fungus strain, a paclitaxel fermentation production period is short and a cost is low. A HPLC analysis detection result shows that through the shake flask fermentation of the endophytic fungus strain, a paclitaxel |
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