Method, database and primer pair for identifying herbal medicinal materials

The invention provides a method for identifying types of herbal medicinal materials. The method comprises the following steps: obtaining a DNA sample of a herbal medicinal material to be detected; (b) performing a polymerase chain reaction between the DNA sample of the herbal medicinal material to b...

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Bibliographische Detailangaben
Hauptverfasser: LI CHENGYU, WEI PEIXUAN, QIU SHUJIAO, TANG RUISHUANG, CHEN HUILING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a method for identifying types of herbal medicinal materials. The method comprises the following steps: obtaining a DNA sample of a herbal medicinal material to be detected; (b) performing a polymerase chain reaction between the DNA sample of the herbal medicinal material to be detected and at least one primer pair to obtain a polymerase chain reaction product; (c) performing high-resolution melting analysis on the polymerase chain reaction product to obtain a high-resolution melting analysis spectrum of the herbal medicinal material to be detected; and (d) comparing the high-resolution melting analysis spectrum of the herbal medicinal material to be detected with a high-resolution melting analysis spectrum of a known herbal medicinal material.