Moniliformin euzymelinked immunodetection kit

The invention provides a moniliformin euzymelinked immunodetection kit which has the advantages of rapid, sensitive and accurate detection and convenient operation, and is suitable for detecting a large batch of samples. The invention further provides preparation and detection methods of the kit. The kit is characterized in that the kit comprises a coated plate, a moniliformin standard product, a moniliformin single antifreeze dried product and an enzyme-labeled goat anti mouse antibody freeze-dried product; when detection is carried out, the coated plate is taken; the moniliformin standard product of 50 microlitres or a processed sample is added into a micropore; a horseradish peroxidase (HRP)-goat anti mouse antibody of 50 microlitres is added; an antimoniliformin antibody of 50 microlitres is added; reaction is carried out at room temperature for 30min; washing is carried out 3 times to 5 times by using washing liquid; pat dry is carried out by using absorbent paper; color developing liquid A of 50 microli