Multi-color fluorescent PCR detection kit for nucleic acid testing and genetic typing of hepatitis c virus and application

The invention discloses a multi-color fluorescent PCR kit for nucleic acid testing and genetic typing of hepatitis c virus and an application thereof. The kit comprises RT-PCR reaction liquid, enzyme mixed liquid, HCV reaction liquid I, HCV reaction liquid II and HCV reaction liquid III, and the kit preferably also comprises HCV typing negative control, HCV typing positive control and application instruction. For the extracted HCV RNA, the hepatitis c virus RNA and the subtype of the hepatitis c virus in a test sample can be rapidly detected by adopting three-tube multi-color fluorescent PCR through one-step RT-PCR. The amplification method of the kit is simple, simplicity in operation is realized, the testing result is high in sensitivity, the specificity is good, the kit can be used for testing and genetic typing of HCV RNA in human serum or plasma, the judgment on the treatment difficulty can be favored, the establishment of an individual antivirus treatment scheme is favored, and the kit has the clinical popularization value.