Epothilone B separating and extracting method based on molecularly imprinted membrane filtering technology

The invention discloses an epothilone B separating and extracting method based on the molecularly imprinted membrane filtering technology. The method comprises the following steps: fermentation liquid is adsorbed by macroporous resin and resolved by ethyl acetate to obtain a crude product; the crude product is filtered by a micro-filtration membrane and an ultrafiltration membrane to remove macromolecules and other impurities; filtrate is filtered by the epothilone B molecularly imprinted membrane to obtain pure epothilone B liquid; and finally, the pure epothilone B liquid is obtained through crystallization. The method is convenient for continuous operation, is easy to enlarge, simple to operate, low in cost and high in selectivity, can rapidly separate the epothilone B molecules from a mixture of structural analogues, and can be put into industrial production easily.