ELISA (enzyme linked immunosorbent assay) diagnostic kit for thymidine kinase (TK)

The invention provides an ELISA (enzyme linked immunosorbent assay) diagnostic kit for thymidine kinase (TK). A sample testing solution, a negative control and a positive control are arranged in the kit, wherein the sample testing solution adopts specific antigen polypeptide. The invention discloses a preparation method of molecular specific antigen polypeptide for breast cancer diagnosis novel moleculeTK1 (1-205) specific antigen polypeptide diagnosed through breast cancer, fusion protein containing the polypeptide, coding DNA, a recombinant vector carrying the DNA, a transformant containing the recombinant vector, an antibody generation method, a method or a reagent for detecting or determining an antibody, a method and a reagent for breast cancer diagnosis. The ELISA diagnostic kit for TK can be used for expression of a recombinant antigen, diagnosis of breast cancer and other malignant tumors, and particularly for development of an early diagnostic reagent for in vitro breast cancer.