Method for fast detecting BRAF gene mutation with allele RNA (ribonucleic acid) isothermal amplification method

A method for fast detecting BRAF gene mutation with an allele RNA (ribonucleic acid) isothermal amplification method comprises the following steps: cell lysis treatment is performed; RNA reverse transcription treatment is performed; cDNA (complementary deoxyribonucleic acid) is generated and serves as an RNA amplification template; and RNA amplicon is quantified through regular monitoring for a fluorescence signal. According to the method, an isothermal reaction method is adopted for fixed-point specific detection of a V600E mutation site of the BRAF, the detection is performed at the isothermal temperature of 41 DEG C, test conditions are mild, chemical compounds for reaction are common, the operation is simple, the specificity is high, and only an instrument capable of detecting a fluorescent probe is required. A higher amplification coefficient is provided, a billion RNAs which are complementary with the target RNA are produced with two specific primers aiming at targeted RNA as well as three enzymes in 1.