Chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment and expression and application thereof

The invention relates to a chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment, as well as expression and application thereof, belonging to the fields of genetic engineering technologies, antibodies and kits. According to the chemically synthesized staphylococcus aureus...

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Hauptverfasser: LI SUQIN, XU YUEYUE, ZHOU JIE, CHEN LERU, ZHANG SUFEN, YUAN JINGYU, PAN YING, SHANG YANHONG, LI YUEXI, LI BINGJUN, XU GUILI, MA YING, CAI RAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment, as well as expression and application thereof, belonging to the fields of genetic engineering technologies, antibodies and kits. According to the chemically synthesized staphylococcus aureus surface protein FnBPA gene fragment provided by the invention, a strong antigen epitope, namely 133 amino acids in total from 745th amino acid to 877th amino acid in the staphylococcus aureus surface protein FnBPA are screened out through computer analysis, a codon preferred by prokaryotes is selected, a brand new gene sequence of the antigen epitope is chemically synthesized, the gene fragment is expressed by utilizing the genetic engineering technology, and the strong antigen epitope fragment of the staphylococcus aureus surface protein FnBPA is prepared. The expressed strong antigen epitope fragment of the staphylococcus aureus surface protein FnBPA can be used for detection of staphylococcus aureus antibodies a