New extraction method in crude extraction of recombinant human growth hormone

The invention relates to a new extraction method in crude extraction of a recombinant human growth hormone, which is used for the crude extraction stage of the recombinant human growth hormone. The new extraction method comprises the steps of preparing a 20mM phosphate buffer (PB), wherein the buffer comprises disodium hydrogen phosphate, sodium dihydrogen phosphate, EDTA (ethylenediaminetetraacetic acid) and purified water in the proportion of 5.732g: 0.624g: 0.292g: 1000ml; adding thalli into a buffer system for pyrolysis; centrifuging and collecting supernatant liquid; adding gradient ammonium sulfate into the supernatant liquid for salt precipitation, centrifuging and collecting a centrifugal precipitate; dissolving the centrifugal precipitate with a de-buffer solution, and centrifuging to obtain the protein-containing supernatant liquid to complete the crude extraction. The new extraction method provided by the invention has the positive effects that by using the bacterium-breaking buffer system to perfo