Babesiidae protozoon detection primer and real-time fluorescent quantitative PCR (polymerase chain reaction) kit

The invention relates to detection of a pair of babesiidae protozoon detection primer, and the upstream and downstream primer sequences are shown as SEQ ID No.1 and SEQ ID No.2. A real-time fluorescent PCR (polymerase chain reaction) kit for babesiidae protozoon detection comprises a Taq (thermu aquaticus) DNA polymerase mixed liquid, a ROX (roxithromycin) dye, a primer 1 with a nucleotide sequence shown as the SEQ ID No.1, a primer 2 with a nucleotide sequence shown as the SEQ ID No.2, a TaqMan probe with a nucleotide sequence shown as SEQ ID No.3, a negative control and a positive control. The provided real-time fluorescent quantitative PCR detection kit is convenient to use, is less in reagent use amount, greatly simplifies the operation process, reduces pollution in the operation process, is strong in detection effect specificity and is high in sensitivity.