Ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit

The invention relates to an ivermectin detection kit, in particular to an ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit. The ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit comprises a kit body, an elisa plate arranged in the kit body and a rea...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: WANG SHANPU, ZHENG MING, GUO HONGWEI, JIN WANXIA, WU TINGCAI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to an ivermectin detection kit, in particular to an ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit. The ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit comprises a kit body, an elisa plate arranged in the kit body and a reagent filled in the kit body, wherein each hole of the elisa plate is coated with coating antigens, the coating antigens are prepared by coupling metabolites of ivermectin with ovalbumin; the reagent comprises monoclonal antibodies of the ivermectin, elisa secondary antibodies, namely horseradish peroxidase-marked goat-anti-mouse antibodies, ivermectin series of standard solutions, concentrated phosphate buffer liquid, concentrated washing liquid and luminescent liquid. By using the kit, due to the adoption of the steps of adding a sample, washing, adding the elisa secondary antibodies, washing, adding the luminescent liquid, detecting, and judging results, the prepared kit has high sensitivity which can reach 0.01