Dual RT-PCR detection method for corn lethal necrosis

A dual RT-PCR detection method for the corn lethal necrosis comprises the following steps: 1), extracting RNA of a sample: extracting an RNA of a sample to be detected; 2), carrying out an inverse transcription reaction on the total RNA of the sample; 3), carrying out dual polymerase chain reactions...

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Bibliographische Detailangaben
Hauptverfasser:	DING YUANMING, LI MIN, DUAN LUHUA, ZHAO MINGFU, LEI QUWEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A dual RT-PCR detection method for the corn lethal necrosis comprises the following steps: 1), extracting RNA of a sample: extracting an RNA of a sample to be detected; 2), carrying out an inverse transcription reaction on the total RNA of the sample; 3), carrying out dual polymerase chain reactions by specific primers of a sugarcane mosaic virus and a maize chlorotic mottle virus at the same time, wherein the specific primers are SCMV1F, SCMV2R, MCMV3333F and MCMV4173R; 4) carrying out electrophoresis detection on the PCR reaction products; 5) judging whether the sample carries the two viruses.