Real-time fluorescent PCR (polymerase chain reaction) kit and oligonucleotides for identifying zygosaccharomyces rouxii

The invention discloses a real-time fluorescent PCR (polymerase chain reaction) kit and oligonucleotides for identifying zygosaccharomyces rouxii. Specifically, the invention provides a group of oligonucleotides for identifying the zygosaccharomyces rouxii. The roup of oligonucleotides are shown in...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN SHIQIONG, GUO ZHENGLEI, RAO HONG, CAI XUEFENG, PANG BO, FU PUBO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a real-time fluorescent PCR (polymerase chain reaction) kit and oligonucleotides for identifying zygosaccharomyces rouxii. Specifically, the invention provides a group of oligonucleotides for identifying the zygosaccharomyces rouxii. The roup of oligonucleotides are shown in sequence tables from SEQ ID No:1 to SEQ ID No:3, wherein the sequences of SEQ ID NO:1 and SEQ ID NO:2 respectively are an upstream primer and a downstream primer for identifying the zygosaccharomyces rouxii, and the sequence of SEQ ID NO:3 is a fluorescent probe for identifying the zygosaccharomyces rouxii. The invention also provides the real-time fluorescent PCR (polymerase chain reaction) kit containing the primers and the probe and a real-time fluorescent PCR identification method of the zygosaccharomyces rouxii in foods. The identification sensitivity of the kit and oligonucleotides disclosed by the invention is within 10-4ng/microliter.