Two-color fluorogenic quantitative PCR (Polymerase Chain Reaction) detection kit for CDV (canine distemper viruses) and CPV (canine parvo viruses)

The invention discloses a two-color fluorogenic quantitative PCR (Polymerase Chain Reaction) detection kit for CDV (canine distemper viruses) and CPV (canine parvo viruses). A detection method comprises the following steps: firstly, pre-treating a sample by using a nucleic acid extraction solution o...

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Bibliographische Detailangaben
Hauptverfasser:	JIA CHUNLING, WEI WENKANG, YUAN JIE, ZHOU XIURONG, CHEN QINLING, TAN WANHONG, ZHAI SHAOLUN, LIN LIFENG, WEN XIAOHUI, XU GUIFENG, LYU DIANHONG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a two-color fluorogenic quantitative PCR (Polymerase Chain Reaction) detection kit for CDV (canine distemper viruses) and CPV (canine parvo viruses). A detection method comprises the following steps: firstly, pre-treating a sample by using a nucleic acid extraction solution of the kit and extracting nucleic acid; then detecting the nucleic acids of the CDV and CPV simultaneously in a reaction system by utilizing the two-color fluorogenic quantitative PCR detection kit, and detecting whether the canine distemper and canine parvo viruses exist in the sample to be detected or not by one step; and quantifying a virus copy number. The detection method is high in detection efficiency and good in specificity and sensibility; the aims of clinical detection and epidemic situation control of animal epidemic diseases can be realized.