Plumbago auriculata rapid propagation technology

The invention relates to the technical field of plant cultivation, and especially relates to the technical field of plumbago auriculata tissue culturing. The technical scheme provided by the invention is that a propagation technology for inducing cluster bud of plumbago auriculata from single bud. T...

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Bibliographische Detailangaben
Hauptverfasser: CHEN KAI, ZHOU LINGYUN, LEI TING, WU PEIWEN, ZHANG SHUO, WEN JINYAN, GAO SUPING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of plant cultivation, and especially relates to the technical field of plumbago auriculata tissue culturing. The technical scheme provided by the invention is that a propagation technology for inducing cluster bud of plumbago auriculata from single bud. The propagation method comprises that after processed, a plumbago auriculata tender stem segment is successively inoculated to a single-bud-induced cluster bud culture medium with a formula of MS+0.2 mg/L-1.0 mg/L 6-BA+1.0 mg/L-4.0 mg/L 2, 4-D and a rooting culture medium with a formula of MS+0.5 mg/L-1.5 mg/L IBA+0.0 mg/L-1.0 mg/L NAA, wherein the culture temperature is 20-28 DEG C, the illuminance is 1500-2500 Lx, the illumination time is 8-12 h/d and the sucrose concentration is 30 g/L; and the culture mediums are solidified by agar with a concentration of 6.5 g/L, and the pH value of the culture mediums is adjusted to 5.8 by NaOH or HCl with a concentration of 0.1 mol/L. Plumbago auriculata cultured by employing