Kit for detection of Yersinia pestis through three-color fluorescent quantification PCR and detection method

The invention relates to a kit for detection of Yersinia pestis nucleic acids and a detection method, and belongs to the biological technology field. The reagents comprises a nucleic acid extracting solution, a Taq enzyme system, fluorescent quantification PCR MIX, positive quality controls and negative reference substances. The detection method employs the three-color fluorescent quantification PCR technology, and through one amplification test, pla and caf1 genes in the plasmids and 3a gene in the chromosomes of Yersinia pestis in samples can be detected qualitatively and quantitatively at the same time. The method comprises the following steps: pretreatment of samples; extraction of nucleic acids; three-color fluorescent quantification PCR detection; analysis of detection results; determination of existence or not of Yersinia pestis in samples to be detected and precise quantification. The detection kit and method have high sensitivity and good specificity, and the method is rapid and efficient. The purpos