Livestock chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method

The invention discloses livestock chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method.. The kit is designed with two primers and one probe in one region according to the MOMP on target sequences, and can specially...

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Bibliographische Detailangaben
Hauptverfasser:	WANG GUOMIN, WANG YU, LI YINGGUO, YANG JUN, NIE FUPING, YUAN ZENGZHUANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses livestock chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method.. The kit is designed with two primers and one probe in one region according to the MOMP on target sequences, and can specially amplify livestock chlamydia. The kit comprises an amplification reaction solution, a positive control, a negative control and sterilized deionized water. The method can quickly, efficiently, specifically and sensitively detect the target sequences by a two-step amplification process under simple reaction conditions, is simple to operate, does not need any expensive instrument or reagent, and has the advantages of no technical requirements for operating personnel, low detection cost and short detection time.