Method for preparing plant root growth promoting agent

The invention provides a method for preparing a plant root growth promoting agent, and belongs to the technical field of biology. Lentinan has various beneficial effects on animals and plants. The method comprises the following steps of preparing high-activity hyphae from lentinus edodes mushroom an...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: ZHANG GUOYUN, HAN YANG, LI XINGLIN, HAN GUANGJIAN, ZHAO NA, CAO AIJIA, CAO XUEFEI, JIA SHIRU
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator ZHANG GUOYUN
HAN YANG
LI XINGLIN
HAN GUANGJIAN
ZHAO NA
CAO AIJIA
CAO XUEFEI
JIA SHIRU
description The invention provides a method for preparing a plant root growth promoting agent, and belongs to the technical field of biology. Lentinan has various beneficial effects on animals and plants. The method comprises the following steps of preparing high-activity hyphae from lentinus edodes mushroom and the like, carrying out mutagenesis of the hyphae, and primarily screening acid-proof hypha mutants orderly through an acidic liquid culture medium and a perlite solid culture medium; rescreening through multiple subculture by virtue of the acidic culture medium and typical antagonism testing to obtain acid-proof mutants; forming the acid-proof mutants into a well dispersed cell line through shaking culture; optimizing the culture process and carrying out deep open culture in a culture medium having the pH in the range from 2.0 to 4.0; collecting a filtrate from the fermentation liquid, and then carrying out hot water extraction to obtain crude polysaccharide of lentinus edodes, deproteinization by Sevag method, H
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN103535352A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN103535352A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN103535352A3</originalsourceid><addsrcrecordid>eNrjZDDzTS3JyE9RSMsvUigoSi1ILMrMS1coyEnMK1Eoys8vUUgvyi8vyQDK5efml4DkEtNT80p4GFjTEnOKU3mhNDeDoptriLOHbmpBfnxqcUFicmpeakm8s5-hgbEpCBo5GhOjBgC6yy3P</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for preparing plant root growth promoting agent</title><source>esp@cenet</source><creator>ZHANG GUOYUN ; HAN YANG ; LI XINGLIN ; HAN GUANGJIAN ; ZHAO NA ; CAO AIJIA ; CAO XUEFEI ; JIA SHIRU</creator><creatorcontrib>ZHANG GUOYUN ; HAN YANG ; LI XINGLIN ; HAN GUANGJIAN ; ZHAO NA ; CAO AIJIA ; CAO XUEFEI ; JIA SHIRU</creatorcontrib><description>The invention provides a method for preparing a plant root growth promoting agent, and belongs to the technical field of biology. Lentinan has various beneficial effects on animals and plants. The method comprises the following steps of preparing high-activity hyphae from lentinus edodes mushroom and the like, carrying out mutagenesis of the hyphae, and primarily screening acid-proof hypha mutants orderly through an acidic liquid culture medium and a perlite solid culture medium; rescreening through multiple subculture by virtue of the acidic culture medium and typical antagonism testing to obtain acid-proof mutants; forming the acid-proof mutants into a well dispersed cell line through shaking culture; optimizing the culture process and carrying out deep open culture in a culture medium having the pH in the range from 2.0 to 4.0; collecting a filtrate from the fermentation liquid, and then carrying out hot water extraction to obtain crude polysaccharide of lentinus edodes, deproteinization by Sevag method, H</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PEST REPELLANTS OR ATTRACTANTS ; PLANT GROWTH REGULATORS ; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20140129&amp;DB=EPODOC&amp;CC=CN&amp;NR=103535352A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20140129&amp;DB=EPODOC&amp;CC=CN&amp;NR=103535352A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG GUOYUN</creatorcontrib><creatorcontrib>HAN YANG</creatorcontrib><creatorcontrib>LI XINGLIN</creatorcontrib><creatorcontrib>HAN GUANGJIAN</creatorcontrib><creatorcontrib>ZHAO NA</creatorcontrib><creatorcontrib>CAO AIJIA</creatorcontrib><creatorcontrib>CAO XUEFEI</creatorcontrib><creatorcontrib>JIA SHIRU</creatorcontrib><title>Method for preparing plant root growth promoting agent</title><description>The invention provides a method for preparing a plant root growth promoting agent, and belongs to the technical field of biology. Lentinan has various beneficial effects on animals and plants. The method comprises the following steps of preparing high-activity hyphae from lentinus edodes mushroom and the like, carrying out mutagenesis of the hyphae, and primarily screening acid-proof hypha mutants orderly through an acidic liquid culture medium and a perlite solid culture medium; rescreening through multiple subculture by virtue of the acidic culture medium and typical antagonism testing to obtain acid-proof mutants; forming the acid-proof mutants into a well dispersed cell line through shaking culture; optimizing the culture process and carrying out deep open culture in a culture medium having the pH in the range from 2.0 to 4.0; collecting a filtrate from the fermentation liquid, and then carrying out hot water extraction to obtain crude polysaccharide of lentinus edodes, deproteinization by Sevag method, H</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</subject><subject>CHEMISTRY</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PEST REPELLANTS OR ATTRACTANTS</subject><subject>PLANT GROWTH REGULATORS</subject><subject>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2014</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDDzTS3JyE9RSMsvUigoSi1ILMrMS1coyEnMK1Eoys8vUUgvyi8vyQDK5efml4DkEtNT80p4GFjTEnOKU3mhNDeDoptriLOHbmpBfnxqcUFicmpeakm8s5-hgbEpCBo5GhOjBgC6yy3P</recordid><startdate>20140129</startdate><enddate>20140129</enddate><creator>ZHANG GUOYUN</creator><creator>HAN YANG</creator><creator>LI XINGLIN</creator><creator>HAN GUANGJIAN</creator><creator>ZHAO NA</creator><creator>CAO AIJIA</creator><creator>CAO XUEFEI</creator><creator>JIA SHIRU</creator><scope>EVB</scope></search><sort><creationdate>20140129</creationdate><title>Method for preparing plant root growth promoting agent</title><author>ZHANG GUOYUN ; HAN YANG ; LI XINGLIN ; HAN GUANGJIAN ; ZHAO NA ; CAO AIJIA ; CAO XUEFEI ; JIA SHIRU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN103535352A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2014</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES</topic><topic>CHEMISTRY</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PEST REPELLANTS OR ATTRACTANTS</topic><topic>PLANT GROWTH REGULATORS</topic><topic>PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHANG GUOYUN</creatorcontrib><creatorcontrib>HAN YANG</creatorcontrib><creatorcontrib>LI XINGLIN</creatorcontrib><creatorcontrib>HAN GUANGJIAN</creatorcontrib><creatorcontrib>ZHAO NA</creatorcontrib><creatorcontrib>CAO AIJIA</creatorcontrib><creatorcontrib>CAO XUEFEI</creatorcontrib><creatorcontrib>JIA SHIRU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHANG GUOYUN</au><au>HAN YANG</au><au>LI XINGLIN</au><au>HAN GUANGJIAN</au><au>ZHAO NA</au><au>CAO AIJIA</au><au>CAO XUEFEI</au><au>JIA SHIRU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for preparing plant root growth promoting agent</title><date>2014-01-29</date><risdate>2014</risdate><abstract>The invention provides a method for preparing a plant root growth promoting agent, and belongs to the technical field of biology. Lentinan has various beneficial effects on animals and plants. The method comprises the following steps of preparing high-activity hyphae from lentinus edodes mushroom and the like, carrying out mutagenesis of the hyphae, and primarily screening acid-proof hypha mutants orderly through an acidic liquid culture medium and a perlite solid culture medium; rescreening through multiple subculture by virtue of the acidic culture medium and typical antagonism testing to obtain acid-proof mutants; forming the acid-proof mutants into a well dispersed cell line through shaking culture; optimizing the culture process and carrying out deep open culture in a culture medium having the pH in the range from 2.0 to 4.0; collecting a filtrate from the fermentation liquid, and then carrying out hot water extraction to obtain crude polysaccharide of lentinus edodes, deproteinization by Sevag method, H</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN103535352A
source esp@cenet
subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
SPIRITS
TRAPPING
VINEGAR
WINE
title Method for preparing plant root growth promoting agent
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T18%3A44%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=ZHANG%20GUOYUN&rft.date=2014-01-29&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN103535352A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true