Method for preparing plant root growth promoting agent

The invention provides a method for preparing a plant root growth promoting agent, and belongs to the technical field of biology. Lentinan has various beneficial effects on animals and plants. The method comprises the following steps of preparing high-activity hyphae from lentinus edodes mushroom an...

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Hauptverfasser:	ZHANG GUOYUN, HAN YANG, LI XINGLIN, HAN GUANGJIAN, ZHAO NA, CAO AIJIA, CAO XUEFEI, JIA SHIRU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a method for preparing a plant root growth promoting agent, and belongs to the technical field of biology. Lentinan has various beneficial effects on animals and plants. The method comprises the following steps of preparing high-activity hyphae from lentinus edodes mushroom and the like, carrying out mutagenesis of the hyphae, and primarily screening acid-proof hypha mutants orderly through an acidic liquid culture medium and a perlite solid culture medium; rescreening through multiple subculture by virtue of the acidic culture medium and typical antagonism testing to obtain acid-proof mutants; forming the acid-proof mutants into a well dispersed cell line through shaking culture; optimizing the culture process and carrying out deep open culture in a culture medium having the pH in the range from 2.0 to 4.0; collecting a filtrate from the fermentation liquid, and then carrying out hot water extraction to obtain crude polysaccharide of lentinus edodes, deproteinization by Sevag method, H