Potato virus-free seedling tissue culture propagation method

The invention discloses a potato virus-free seedling tissue culture propagation method which relates to the propagation technology of potatoes and belongs to the technical filed of agriculture planting. The method is characterized by comprising the following steps: performing fine selection, performing accelerating germination, performing seed airing, and inoculating aired seeds in a germination culture medium for cultivation and growth, wherein the accelerating germination and sowing are performed at the temperature being 15-20 DEG C; the method has the beneficial effects that compared with a formula based on an MS, a formula in the method is small in quantity of constitution chemical substances, low in cost, and convenient to prepare. By using the method disclosed by the invention, potato virus-free seedlings can be propagated in an opening environment, and the characteristics that the propagation speed is high, the production cost is low, the popularization and the application are easy, and the like can be