AlphaLISA detection kit of penicillinase in milk product

The invention provides an AlphaLISA detection kit of penicillinase in a milk product, and the AlphaLISA detection kit comprises a penicillinase standard product, biotin penicillinase, anti-penicillinase monoclonal antibody, an anti-mouse-IgG (immunoglobulin G) coated receptor magnetic bead, a streptavidin donor magnetic bead, a detection buffer solution, a white reaction plate, a sealing membrane and an operating specification. The anti-mouse-IgG coated receptor magnetic bead includes 100 mug / mL of a receptor magnetic bead, 0.05% of Proclin-300, and a phosphate buffer solution (PBS) with the PH of 7.4, and the streptavidin donor magnetic bead includes 100 mug / mL of a donor bead, 25mM of HEPES, 100mM of a NaCl solution and 0.05% of the Proclin-300. An AlphaLISA detection method of the penicillinase in the milk product is established by use of the AlphaLISA detection kit, and the method has the advantages of less sample use amount, high sensitivity, no washing, short detecting period, and the like.