Method for identifying DNA barcodes of three gentiana macrophylla medicinal materials in pharmacopeia

The invention discloses a method for identifying DNA (Deoxyribose Nucleic Acid) barcodes of three gentiana macrophylla medicinal materials in the pharmacopeia. Three gentian macrophylla medicinal materials in the pharmacopeia are respectively Gentiana macrophylla Pall, G.crassicaulis Duthie, and G.s...

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Bibliographische Detailangaben
Hauptverfasser:	MENG MEI, HUANG LUQI, ZHANG MINGYAN, MA ZONGHUA, LIU SHOUJIN, YANG QINGSHAN, PENG HUASHENG, FANG CHENGWU, LI QINGLIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for identifying DNA (Deoxyribose Nucleic Acid) barcodes of three gentiana macrophylla medicinal materials in the pharmacopeia. Three gentian macrophylla medicinal materials in the pharmacopeia are respectively Gentiana macrophylla Pall, G.crassicaulis Duthie, and G.straminea Maxim. The identification method comprises two steps of carrying out DNA extraction and splicing obtained DNA sequences, wherein in the DNA extraction step, a good extraction effect is obtained by prolonging cracking heating time and utilizing phenol and trichloromethane, between which the volume ratio is 1:1, to remove interference of phenol and polysaccharide substances; then the obtained DNA sequences are spliced to identify gentiana macrophylla so as to obtain a good identifying effect.