Method for preparing neem cell culture insecticide
The invention discloses a method for preparing a neem cell culture insecticide and belongs to the technical field of biology. Azadirachtin has an effect of controlling multiple injurious insects and is generally extracted from seed kernels of neem trees. Therefore, the azadirachtin production is gre...
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| creator | HAN YANG ZHANG GUOYUN LI XINGLIN ZHAO NA HAN GUANGJIAN CHENG FANG CAO AIJIA CAO XUEFEI JIA SHIRU |
| description | The invention discloses a method for preparing a neem cell culture insecticide and belongs to the technical field of biology. Azadirachtin has an effect of controlling multiple injurious insects and is generally extracted from seed kernels of neem trees. Therefore, the azadirachtin production is greatly limited. The defects in the aspect can be overcome by adopting industrial production of neem cell culture. The method comprises the following steps: inducing and performing subculture to obtain loose neem callus; performing shaking culture, screening and subculture to form a cell line with high dispersibility; performing induction, shaking culture, screening and identifying to obtain small cell mutants; optimizing suspension cultures and culture conditions of the small cell mutants, and ensuring that the azadirachtin can be secreted outside the cells; and obtaining a product prepared from four extracting solutions from the callus and the suspension cultures. The products have obvious insect control effects. Th |
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Azadirachtin has an effect of controlling multiple injurious insects and is generally extracted from seed kernels of neem trees. Therefore, the azadirachtin production is greatly limited. The defects in the aspect can be overcome by adopting industrial production of neem cell culture. The method comprises the following steps: inducing and performing subculture to obtain loose neem callus; performing shaking culture, screening and subculture to form a cell line with high dispersibility; performing induction, shaking culture, screening and identifying to obtain small cell mutants; optimizing suspension cultures and culture conditions of the small cell mutants, and ensuring that the azadirachtin can be secreted outside the cells; and obtaining a product prepared from four extracting solutions from the callus and the suspension cultures. The products have obvious insect control effects. Th</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; PEST REPELLANTS OR ATTRACTANTS ; PLANT GROWTH REGULATORS ; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF ; TRAPPING</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140108&DB=EPODOC&CC=CN&NR=103493844A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140108&DB=EPODOC&CC=CN&NR=103493844A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HAN YANG</creatorcontrib><creatorcontrib>ZHANG GUOYUN</creatorcontrib><creatorcontrib>LI XINGLIN</creatorcontrib><creatorcontrib>ZHAO NA</creatorcontrib><creatorcontrib>HAN GUANGJIAN</creatorcontrib><creatorcontrib>CHENG FANG</creatorcontrib><creatorcontrib>CAO AIJIA</creatorcontrib><creatorcontrib>CAO XUEFEI</creatorcontrib><creatorcontrib>JIA SHIRU</creatorcontrib><title>Method for preparing neem cell culture insecticide</title><description>The invention discloses a method for preparing a neem cell culture insecticide and belongs to the technical field of biology. Azadirachtin has an effect of controlling multiple injurious insects and is generally extracted from seed kernels of neem trees. Therefore, the azadirachtin production is greatly limited. The defects in the aspect can be overcome by adopting industrial production of neem cell culture. The method comprises the following steps: inducing and performing subculture to obtain loose neem callus; performing shaking culture, screening and subculture to form a cell line with high dispersibility; performing induction, shaking culture, screening and identifying to obtain small cell mutants; optimizing suspension cultures and culture conditions of the small cell mutants, and ensuring that the azadirachtin can be secreted outside the cells; and obtaining a product prepared from four extracting solutions from the callus and the suspension cultures. The products have obvious insect control effects. 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Azadirachtin has an effect of controlling multiple injurious insects and is generally extracted from seed kernels of neem trees. Therefore, the azadirachtin production is greatly limited. The defects in the aspect can be overcome by adopting industrial production of neem cell culture. The method comprises the following steps: inducing and performing subculture to obtain loose neem callus; performing shaking culture, screening and subculture to form a cell line with high dispersibility; performing induction, shaking culture, screening and identifying to obtain small cell mutants; optimizing suspension cultures and culture conditions of the small cell mutants, and ensuring that the azadirachtin can be secreted outside the cells; and obtaining a product prepared from four extracting solutions from the callus and the suspension cultures. The products have obvious insect control effects. Th</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES FISHING FORESTRY HUMAN NECESSITIES HUNTING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF TRAPPING |
| title | Method for preparing neem cell culture insecticide |
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