Method for preparing neem cell culture insecticide

The invention discloses a method for preparing a neem cell culture insecticide and belongs to the technical field of biology. Azadirachtin has an effect of controlling multiple injurious insects and is generally extracted from seed kernels of neem trees. Therefore, the azadirachtin production is greatly limited. The defects in the aspect can be overcome by adopting industrial production of neem cell culture. The method comprises the following steps: inducing and performing subculture to obtain loose neem callus; performing shaking culture, screening and subculture to form a cell line with high dispersibility; performing induction, shaking culture, screening and identifying to obtain small cell mutants; optimizing suspension cultures and culture conditions of the small cell mutants, and ensuring that the azadirachtin can be secreted outside the cells; and obtaining a product prepared from four extracting solutions from the callus and the suspension cultures. The products have obvious insect control effects. Th