Preparation method of high quality blood cell protein peptide by using two-step enzymolysis method

The invention discloses a preparation method of high quality blood cell protein peptide by using a two-step enzymolysis method, and the preparation method comprises adding an appropriate amount of sodium citrate in fresh pig blood for anticoagulation, using an industrial tubular centrifuge for high-speed centrifugal separation to obtain fresh red blood cells; adding an appropriate amount of water in the fresh red blood cells, regulating pH, adding an appropriate amount of acidic protease S1 for enzymolysis, centrifuging, and removing the centrifugal sedimentation part to obtain a first enzymolysis decolored solution; adding an appropriate amount of neutral protease S2 in the first enzymolysis decolored solution for enzymolysis, adding a very small amount of a harmless non-residual chemical decoloring agent into a secondary enzymatic hydrolysate, heating up to 55-60 DEG C and keeping warm for 3 h to obtain a secondary enzymolysis decolored solution; and performing spray drying of the secondary enzymolysis deco