Ex-vitro rooting method for tissue culture seedlings of pinus massoniana

The invention discloses an ex-vitro rooting method for tissue culture seedlings of pinus massoniana. The ex-vitro rooting method comprises the following steps: taking terminal buds of aseptic seedlings with 0.5cm long hypocotyls as explants for inducing cluster buds in a culture medium I; carrying out elongation culture on the induced cluster buds in a culture medium II, individually cutting off the terminal buds, and inoculating the cut terminal buds into a culture medium III for propagation to obtain subculture buds I; repeatedly elongating the subculture buds I, individually cutting off the subculture buds I, dividing the cut subculture buds I into terminal buds and bud sections, continuously propagating in a subculture propagation culture medium III to obtain subculture buds II, and repeatedly elongating and propagating the subculture buds II to obtain new propagation subculture buds; and transplanting the individually cut 2.5-3.5cm high subculture buds into a seedling culture cup for ex-vitro rooting, an