Immune colloidal gold test strip for detecting porcine epidemic diarrhea virus as well as preparation method and application thereof

The invention discloses an immune colloidal gold test strip for detecting porcine epidemic diarrhea virus as well as a preparation method and application thereof. The test strip sequentially comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane, an absorbent paper and a PVC (poly vinyl chloride) base plate arranged below and used as an assembling platform according to a connection sequence, wherein the colloid gold pad comprises a glass cellulose membrane of a PEDV (porcine epidemic diarrhea virus) monoclonal antibody adsorbed with colloidal gold marks, the nitrocellulose membrane is provided with a goat rat resisting IgG (immunoglobulin G) polyclonal antibody coated quality control line and a rabbit resisting PEDV S protein polyclonal antibody coated detection line, and the PEDV monoclonal antibody is generated in secretion of hybridoma cells with a preservation number CCTCC C201392. Experiments prove that the test strip has the advantages of strong specificity and good stability; the opera