Real-time fluorescent quantitative PCR (polymerase chain reaction) detection method for three genotypes of human parvovirus B19, as well as universal detection primer, TaqMan probe and kit thereof

The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) detection method which can be used for simultaneously performing qualitative and quantitative detection on three genotypes of human parvovirus B19, as well as a universal detection primer, a TaqMan probe and a kit thereof. The universal detection primer and the TaqMan probe disclosed by the invention can be combined with real-time fluorescent quantitative PCR to realize the purpose of accurately, qualitatively and quantitatively detecting B19 virus DNA (deoxyribonucleic acid) of the three genotypes of a sample to be detected, a formed NAT (nucleic acid testing) detection method against the three genotypes of B19 virus on the basis is obviously better than the existing detection technology, and the real-time fluorescent quantitative PCR detection method has the advantages of high accuracy, low pollution, fast detection speed, low requirements on instruments and equipment, low cost and the like; in addition to clinical