Heavy chain single-domain antibody of type-II dengue fever virus NS1 protein as well as preparation method and application of heavy chain single-domain antibody

Heavy chain single-domain antibody of type-II dengue fever virus NS1 protein as well as preparation method and application of heavy chain single-domain antibody

The invention belongs to the technical field of biology and relates to a heavy chain single-domain antibody of a type-II dengue fever virus NS1 protein as well as a preparation method and application of the heavy chain single-domain antibody. The preparation method comprises the following steps of:...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: WANG JUFANG, MA YI, WANG XIAONING, WANG HAIYING, FA DIMA
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention belongs to the technical field of biology and relates to a heavy chain single-domain antibody of a type-II dengue fever virus NS1 protein as well as a preparation method and application of the heavy chain single-domain antibody. The preparation method comprises the following steps of: biologically screening the recombination expressed dengue fever virus NS1 protein for many times by using an alpacas non-immunity heavy chain single-domain antibody library; picking by using an ELISA (Enzyme Linked Immunosorbent Assay) to obtain a specific bonded phage monoclone, and determining a DNA (Deoxyribose Nucleic Acid) sequence; and then, converting the DNA sequence into escherichia coli to express to obtain the heavy chain single-domain antibody. The heavy chain single-domain antibody obtained through screening can be efficiently expressed in the escherichia coli, can be used for preparing a colloid gold detection kit for detecting type-II dengue fever virus infection, and is higher in detection accuracy.