shRNA for inhibiting colon cancer cell PRPS2 expression, construction method of vector of shRNA, and use of shRNA

The invention relates to the field of biological medicine and provides a shRNA for inhibiting colon cancer cell PRPS2 expression, a construction method of a vector of the shRNA, and a use of the shRNA. The shRNA for inhibiting colon cancer cell PRPS2 expression has a sequence shown in the formula of SEQ ID NO: 1. A target sequence of the shRNA is human PRPS2 mRNA from 422th-440th locus. A template sequence and a linearized vector are recombined into a shRNA eukaryotic expression plasmid for inhibiting colon cancer cell PRPS2 expression. In the invention, the shRNA eukaryotic expression plasmid as the vector is obtained successfully by genetic recombination. The vector can stably and continuously reduce a PRPS2 expression level in a human HCT116 cell, solves the problems of short action time and large toxicity of siRNA chemically synthesized in vitro, and can effectively inhibit HCT116 cell proliferation, cell cycle repression and cell apoptosis. Therefore, the shRNA provides a novel tool for human colon cance