Kit for detecting premature rupture of fetal membranes

The invention discloses a detection kit and particularly relates to a kit for detecting premature rupture of fetal membranes. The kit comprises a nitrocellulose membrane and the following reagents: (A) membrane sealing solution, (B) mouse anti-human soluble intercellular adhesion molecule-1 monoclon...

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Hauptverfasser: HU HUAIZHONG, GUAN XIANGQIAN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a detection kit and particularly relates to a kit for detecting premature rupture of fetal membranes. The kit comprises a nitrocellulose membrane and the following reagents: (A) membrane sealing solution, (B) mouse anti-human soluble intercellular adhesion molecule-1 monoclonal antibody detection solution, (C) washing solution, (D) goat anti-mouse IgG polyclonal antibody detection solution marked by horse radish peroxidase, and (E) chromogenic substrate diaminobenzidine solution. The kit disclosed by the invention detects the premature rupture of the fetal membranes by adopting a qualitative detection manner; the manufacturing cost and the use cost are both obviously reduced; meanwhile, compared with immune chromatography test paper for qualitative detection, the kit can effectively improve the sensitivity and the specificity of detection results of the premature rupture of the fetal membranes, and the sensitivity and the specificity reach more than 99% and more than 96% respectively,