Method for detecting SKA1 gene expression and purpose of siRNA thereof

The invention discloses a siRNA for inhibiting SKA1 gene expression, and the length of the siRNA is 15-27 bp. The invention also discloses a recombinant vector containing siRNA, and the recombinant vector transfects the eukaryotic cell, and the cell with SKA1 gene silencing is obtained. The siRNA se...

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Bibliographische Detailangaben
Hauptverfasser:	YU WENXI, SHEN ZAN, LAO XINYUAN, YAO YANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a siRNA for inhibiting SKA1 gene expression, and the length of the siRNA is 15-27 bp. The invention also discloses a recombinant vector containing siRNA, and the recombinant vector transfects the eukaryotic cell, and the cell with SKA1 gene silencing is obtained. The siRNA sequence can effectively degrade mRNA of SKA1 gene, thereby specifically inhibiting expression of SKA1 gene. The siRNA provided by the invention can be used for preparing medicaments for treating SKA1 gene related disease comprising tumour; the siRNA simultaneously can be used for researching effect of SKA1 gene in drug resistance of tumour, especially has a important meaning for clinic non-operation treatment of osteosarcoma and colorectum malignant tumor. The invention also discloses a reverse transcription PCR primer for effectively detecting SKA1 in tissue or cell, and applications of the primer in tumour diagnosis.