Method for extracting total RNA from leucaena leucocephala leaf tissue

The invention discloses a method for extracting total RNA from leucaena leucocephala leaf tissues. During a leucaena leucocephala leaf tissue total RNA extraction process, 0.9-1.0g of crosslinked polyvinyl pyrrolidone (PVPP) powder is added, such that polysaccharides, proteins, and transparent viscous insoluble substances are comprehensively removed. With the method, the OD260/OD280 ratio of the extracted leucaena leucocephala leaf tissue total RNA is 1.8-2.0. The obtained RNA has high purity and high yield. Also, when the obtained RNA extract liquid is detected by using 1.5% agarose gel denaturation electrophoresis, the obtained strip is clear, RNA integrity is good, and the extracted RNA can be completely used in leucaena leucocephala molecular biology researches. The method has important significance in analyzing leucaena leucocephala gene expression and regulation, gene cloning and function identification, and molecular marker-assisted breeding. The method has high practicality, and good promotion ad appl