Dry-type immunoassay test strip and preparation method and application thereof

The present invention relates to a dry-type immunoassay test strip and a preparation method and application thereof. On a plastic substrate of the test strip is sequentially stuck with a sample pad, a nitrocellulose membrane anda water absorption pad from left to right, wherein the right end of nitrocellulose membrane is provided with a control line and a detection line that are parallel to each other; the left end of nitrocellulose membrane is provided with a tracer particle labeled antibody/ antigen-coated line and a closed protein line I, wherein the closed protein line I is formed by coating closed protein solution I with a nitrocellulose membrane, and the antibody/ antigen-coated line is formed by spraying the tracer particle labeled antibody/ antigen on a closed protein line II, and the closed protein line II is formed by coating closed protein solution II with a nitrocellulose membrane. . The test strip can be applied in the immunoassay based on the double antibody sandwich method or the competitive me