Daptomycin separation and purification method

The present invention discloses a daptomycin separation and purification method, which comprises the following concrete steps: adjusting an acid solution to adjust a daptomycin fermentation broth to obtain a mycelium liquid; adding to an alcohol solution, and collecting an alcohol phase extraction solution; adding an alkali solution to the alcohol phase extraction solution to extract, and collecting a water phase solution; adding active carbon to the water phase solution, adding butanol or n-butanol to extract, and collecting an alcohol extraction solution; adding active carbon to the alcohol extraction solution, and reducing pressure; carrying out suction filtration and vacuum drying to obtain daptomycin crude powder; dissolving the daptomycin crude powder in purified water, and carrying out crystallization and suction filtration to obtain daptomycin wet powder; and dissolving the daptomycin wet powder with purified water, and carrying out secondary crystallization, suction filtration and drying to obtain hi