Escherichia coli expression strain with high conversion efficiency

The invention relates to an escherichia coli expression strain China General Microbiological Culture Collection Center (CGMCC) No. 7123 with high conversion efficiency. The strain is obtained through knocking out an hsdR gene of an encoding I-type restriction enzyme in an escherichia coli heterogeno...

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Hauptverfasser:	LI LING, SHANG GUANGDONG, WANG RUIQING, SHI MUDAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an escherichia coli expression strain China General Microbiological Culture Collection Center (CGMCC) No. 7123 with high conversion efficiency. The strain is obtained through knocking out an hsdR gene of an encoding I-type restriction enzyme in an escherichia coli heterogenous protein expression strain BL21(DE3) genome. The strain does not carry any exogenous base sequence after knocking out the hsdR gene. The conversion efficiency of the strain is 14.5 times that of a BL21(DE3) parent strain. The CGMCC No. 7123 can serve as a general heterogenous protein expression strain and a strain for directly screening expression mutant pool proteins.