Photosensitive dye osmium complex and preparation method thereof, canceration early stage deoxyribonucleic acid (DNA) oxidative damage quick detection kit and detection method

The invention provides a photosensitive dye osmium complex and a preparation method thereof, and a kit and a method for performing quick canceration early stage deoxyribonucleic acid (DNA) oxidative damage detection. The photosensitive dye osmium complex has the structural general formula of LL'...

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Hauptverfasser: PENG XIAOJUN, WANG JITAO, MU DAOZHOU, SUN SHIGUO, CAI MINGZHU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a photosensitive dye osmium complex and a preparation method thereof, and a kit and a method for performing quick canceration early stage deoxyribonucleic acid (DNA) oxidative damage detection. The photosensitive dye osmium complex has the structural general formula of LL'L''Os-Y4, wherein Y is a halide ion, ClO, BF, PF or OTs; L and L' are independently selected from 2,2'-dipyridyl and a derivative thereof, phenanthroline and a derivative thereof and DPPZ; and L'' is a precursor with 2,2'-dipyridyl and 4,4'-dipyridyl. The kit at least comprises a main body compound 8-element cucurbit urils (a component A), the photosensitive dye osmium complex (a component B) and pure water or phosphate and borate buffering solution (a component D). By adopting the kit, even under the condition of no instrumental equipment, a user can individually and quickly detect 8-oxoG under visible light with eyes only by one drop of blood.