Method for pre-evaluating isoelectric focusing electrophoresis test result based on conductivity detection

The invention discloses a method for evaluating whether a protein sample pretreatment effect and experiment operation are correct based on isoelectric focusing electrophoresis conductivity detection in the technical field of biological separation and analysis. The method comprises the following steps of: carrying out isoelectric focusing treatment on pretreated protein samples by a curing pH gradient adhesive tape or a non-curing pH gradient adhesive tape; calculating current at an electromigration stage and a diffusion stage during the treatment period so as to obtain the relationship between conductivity and time at the electromigration stage and the diffusion stage; and further, evaluating whether the protein sample pretreatment desalination effect and the experiment operation are correct according to the conductivity. According to the invention, the method can be used for effectively recognizing the good or bad of the electromigration and diffusion conductivity and the protein sample desalination treatmen