Method for improving integrity of cytoskeletons after freeze thawing of preserved boar sperm

The invention discloses a method for improving the integrity of cytoskeletons after freeze thawing of preserved boar sperm. The method comprises the following steps of: collecting the boar sperm from an adult, healthy and carnal exuberant boar; extracting low density lipoprotein (LDL) from pigeon eggs; and freezing the boar sperm by using the LDL as a freezing protective agent. By adopting the method for improving the integrity of the cytoskeletons after freeze thawing of the preserved boar sperm, 9 percent LDL serving as the freezing protective agent is added, so that the integrity of the cytoskeletons after freeze thawing of the boar sperm is improved, the expression quantity of actin in the cytoskeletons of the boar sperm can be obviously improved, meanwhile, the expression quantity of tubulin is closer to that of fresh sperm, the survival rate, vitality and fertilization rate of the freeze-thawed boar sperm are effectively improved, the requirements of real use are furthest met, and the method has relativ