Method for detecting or performing aided detection on sorghum sudanense nationally checked variety
The invention discloses a method for detecting or performing aided detection on sorghum sudanense nationally checked variety. The invention provides a primer group for detecting and performing aided detection on sorghum sudanense nationally checked variety, wherein the primer group consists of 20 pr...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for detecting or performing aided detection on sorghum sudanense nationally checked variety. The invention provides a primer group for detecting and performing aided detection on sorghum sudanense nationally checked variety, wherein the primer group consists of 20 primer groups comprising the primer group P01-primer group P20. The experiment proves that 20 pairs of SSR primers are screened, the pasture is subjected to polymerase chain reaction (PCR) amplification, the electrophoretogram of 20 products and the electrophoretogram of seven sorghum sudanense nationally checked varieties are subjected to band comparison, and if the electrophoretogram of at least 18 in 20 products is consistent with the band, the pasture can be judged to be in the same variety as the sorghum sudanense in a candidate mode. The sorghum sudanense is identified according to the primers and the method, and whether the pasture to be detected is really in the seven nationally checked varieties can be simpl |
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