Quick test method

The invention relates to a quick test method. Firstly, rice bran is partially degreased, lipoxidase in the rice bran which is partially degreased is extracted with a buffer solution, the lipoxidase extracting solution is added to a prepared phosphate-linoleic acid-polysorbate 20 substrate solution,...

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Bibliographische Detailangaben
Hauptverfasser: JIANG CHAOHUI, LI AOLAN, LEI JUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a quick test method. Firstly, rice bran is partially degreased, lipoxidase in the rice bran which is partially degreased is extracted with a buffer solution, the lipoxidase extracting solution is added to a prepared phosphate-linoleic acid-polysorbate 20 substrate solution, changes of light-absorption photometric values are tested under 234 nm in 5 minutes, meanwhile, the protein content in the lipoxidase extracting solution is tested, and then activity of the lipoxidase in the rice bran can be obtained. According to the method, the activity of the lipoxidase in the rice bran is effectively activated through partial degreasing, the detection limit is raised, the buffer solution, the substrate solution and passivating crude enzyme serve as reference solutions, test errors of the crude enzyme are effectively eliminated, and complicated steps of passivating of the crude enzyme are eliminated. The method is easy and convenient to operate, precise in result, good in reproducibility, capabl