Construction method and application of recombinant lactobacillus acidophilus for expressing glucagon-like peptide-2

The invention discloses a construction method and application of recombinant lactobacillus acidophilus for expressing glucagon-like peptide-2. The construction method comprises the following steps: designing a primer containing specific restriction enzyme digestion site according to gene sequence and expression vector plasmid characteristic of disclosed glucagon-like peptide-2; by using small intestinal mucosa as a material, carrying out RT-PCR (reverse transcription-polymerase chain reaction) to obtain fragment containing glucagon-like peptide-2 gene; connecting the fragment with lactobacillus expression vector plasmid Pmg36e to obtain recombinant plasmid Pmg36e-glp2; transferring the recombinant plasmid in actobacillus acidophilus through an electrotransformation method to obtain recombinant lactobacillus acidophilus for expressing glucagon-like peptide-2; expressing under the induction of nisin; and analyzing and proving that the gene is correctly expressed through SDS-PAGE (sodium dodecyl sulfate polyacry