F0F1-ATPase rotary molecular motor sensor kit for detecting Listeria monocytogenes

An F0F1-ATPase rotary molecular motor sensor kit for detecting Listeria monocytogenes belongs to the field of the rapid detection of food microbes, and mainly solves an overlong time (about 5 days) problem of traditional detection methods. The kit is mainly characterized in that an F0F1-ATPase rotary molecular motor sensor on a chromatophore is utilized, an F0F1-ATP synthase can rapidly rotate, and the coupling between a catalytic site and proton transfer is established through rotating the F0F1-ATP synthase. A prfA probe is connected to the epsilon subunit of the ATP synthase, a sample to be measured and a negative contrast substance are respectively combined with a biosensor, the ATP outputs 20min after the ATP catalytic synthesis are compared, the ATP synthetic outputs can be measured through the amount of H in the environment, and the H amount can be obtained through the fluorescence intensity of H-DHPE. The kit can realize the rapid, sensitive and high-flux detection of the Listeria monocytogenes i