Macroporous agarose microspheres and preparation method thereof

The invention relates to a preparation method and a product of macroporous agarose microspheres. The preparation method is characterized by comprising the following steps: adding a high-concentration surface active agent to an agarose aqueous solution to form a large number of micelles in a water phase; dispersing the water phase into an oil phase; and then preparing the macroporous agarose microspheres according to the characteristic that the micelles of the surface active agent in the water phase can absorb the oil and then swells. The prepared product remains the original mesh shaped gel pores of the agarose gel and also has macropores at hundred-nanometer level (100 to 500nm). The preparation method of the macroporous agarose microspheres, provided by the invention, is simple, and the aperture of the product can be controlled; the prepared macroporous agarose microspheres remain the original excellent performances of the agarose gel and greatly increases the aperture, and are ideal liquid phase chromatogr