Manufacturing method of portable enzyme sensor based on carbon nanometer tube

The invention belongs to the field of equipment for plant protection, particularly relates to a manufacturing method of a portable enzyme sensor based on a carbon nanometer tube, and is mainly used for detecting the concentration of an organic phosphorus pesticide, such as chlorpyrifos. The manufacturing method of the portable enzyme sensor based on the carbon nanometer tube provided by the invention comprises the following steps of: ultrasonically diffusing the carbon nanometer tube after being processed in steps of acidizing, centrifuging, cleaning and the like in a chitosan-acetic acid solution with the concentration of 5%; adding an enzyme into the solution, so as to form a carbon nanometer tube/chitosan/acetylcholin esterase sedimentation solution; and covering a carbon nanometer tube/chitosan/acetylcholin esterase composite material modification layer on the surface of a working electrode of a silk-screen printing gold electrode by using an electro-deposition method, so as to obtain the portable enzyme